Optimizing anoxic biological phosphorus removal in the anaerobic-anoxic sequencing batch reactor (A2 SBR) was observed to depend on two parameters: the amount of added nitrate and the sludge retention time (SRT). The concentration of 120 mg N-NO3 · l-1 in the anoxic medium and the SRT of 15 days were determined as optimal for a complete phosphorus removal in the A2 SBR. The reactor was supplied with synthetic wastewater containing 800 mg COD.l-1 acetic acid, 240 mg N-NH4·l-1 and 30 mg P-PO4·l-1. This study was completed by microscopic observations which revealed three morphological types of phosphate-accumulating bacteria (PAB).

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