The fixed biomass inside porous medium has two layers where biomass yield constants are different from each other when it is cultivated in the chemostat reactor. The biomass fixed inside porous medium is tested to see whether the operation type affected the structure of it. Two kinds of operation method of the reactor were used for the biofilm cultivation. One is the batch reactor. Another is the chemostat reactor. From the kinetic test, it is found that the biofilm fixed in the batch reactor does not have two layers that were observed in the biofilm from the chemostat reactor. Within the experimental conditions for type-1, the result of kinetic tests show homogeneous biofilm characteristics. It can be concluded that the reactor type (batch type or chemostat type) affects the structure of biomass fixed inside porous medium.

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