In order to improve activated sludge plant operation (achieving higher efficiency and cost savings) beside influent and effluent characteristics and working parameters (e.g. dissolved oxygen, total and volatile suspended solids, pH, recirculation flow rate, etc.), the biomass activity should be monitored, the bacteria being responsible for the pollutant degradation. Since conventional cultivation based methods are inadequate to quantify environmental microorganisms (due to scarce number of cultivable microorganisms and time-consuming procedures) several “non-conventional” techniques were applied in this study, in order to compare the obtainable information and their routine feasibility. Different measurements (VSS concentration, Oxygen Uptake Rate, microbial counts by cultural and biomolecular methods – MPN-PCR, ATP content, dehydrogenase activity, microbial cell viability and enzymatic activity) were carried out on mixed liquor samples, taken from a municipal activated sludge plant (440,000 p. e.). The preliminary results of the research are presented in this paper.
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Research Article|
July 01 2002
A comparison among different methods for evaluating the biomass activity in activated sludge systems: preliminary results
G. Andreottola;
G. Andreottola
*Dept. of Civil and Env. Eng., Fac. Engineering, Univ. of Trento. Via Mesiano, 77, I – 38050 Trento
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L. Baldassarre;
L. Baldassarre
**AGSM Verona SpA, Municipal WWTP. Via Avesani, 33, I – 37100 Verona
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C. Collivignarelli;
C. Collivignarelli
***Dept. of Civil Eng., Fac. Engineering, Univ. of Brescia. Via Branze 38, I – 25123 Brescia
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R. Pedrazzani;
R. Pedrazzani
***Dept. of Civil Eng., Fac. Engineering, Univ. of Brescia. Via Branze 38, I – 25123 Brescia
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P. Principi;
P. Principi
****Dept. of Food Sci. and Microbiology, Fac. of Agron. Univ. of Milan. Via Celoria, 2, I – 20133 Milan
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C. Sorlini;
C. Sorlini
****Dept. of Food Sci. and Microbiology, Fac. of Agron. Univ. of Milan. Via Celoria, 2, I – 20133 Milan
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G. Ziglio
G. Ziglio
*Dept. of Civil and Env. Eng., Fac. Engineering, Univ. of Trento. Via Mesiano, 77, I – 38050 Trento
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Water Sci Technol (2002) 46 (1-2): 413–417.
Citation
G. Andreottola, L. Baldassarre, C. Collivignarelli, R. Pedrazzani, P. Principi, C. Sorlini, G. Ziglio; A comparison among different methods for evaluating the biomass activity in activated sludge systems: preliminary results. Water Sci Technol 1 July 2002; 46 (1-2): 413–417. doi: https://doi.org/10.2166/wst.2002.0509
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