Batch experiments were conducted to convert molasses wastewater (10-160 g COD/L) into hydrogen at 35°C at various pH (4-8). The maximum hydrogen productivity (HP) and hydrogen production rate (HPR) reached 47.1 mmol-H2/g CODre and 97.5 mmol-H2/L-day, respectively, at a substrate concentration of 40 g COD/L and pH 6.0. The methane-free biogas contained up to 50% (v/v) of hydrogen. Fermentation at wastewater concentrations higher than 60 g COD/L required a long acclimation period (more than 20 h). Though the fractions were substrate concentration and pH-dependent, acetate and butyrate were the two main liquid fermentation products. A comparison of the HP and HPR data indicates that defining a hydrogen yield indicator to evaluate hydrogen generation efficiency should be taken into consideration in practical fermentation operations.
Biohydrogen production by mesophilic fermentation of food wastewater
J.-H. Wu, C.-Y. Lin; Biohydrogen production by mesophilic fermentation of food wastewater. Water Sci Technol 1 March 2004; 49 (5-6): 223–228. doi: https://doi.org/10.2166/wst.2004.0757
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