Biological denitrification was studied in an SBR to investigate the performance. The experimental results showed that granular sludge with high activity and good settling ability can be developed smoothly in the reactor. With the granular sludge, high nitrate loading rate (0.48 kg NO3-N dm−3 d−1) and removal efficiency (more than 99%) can be achieved. Scanning electronic microscope examination showed that only rod-shape bacteria existed on and within the granules, which confirmed indirectly that filamentous bacteria do not utilize nitrate and nitrite as electron acceptors. SBR is an alternative and effective technology for biological denitrification.

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