This study was conducted to compare the performance of a continuous-flow stirred-tank reactor (CSTR) and an anaerobic sequencing batch reactor (ASBR) for fermentative hydrogen production at various substrate concentrations. Heat-treated anaerobic sludge was utilized as an inoculum, and hydraulic retention time (HRT) for each reactor was maintained at 12h. At the influent sucrose concentration of 5g COD/L, start-up was not successful in both reactors. The CSTR, which was started-up at 10g COD/L, showed stable hydrogen production at the influent sucrose concentrations of 10–60g COD/L during 203 days. Hydrogen production was dependent on substrate concentration, resulting in the highest performance at 30g COD/L. At the lower substrate concentration, the hydrogen yield (based on hexose consumed) decreased with biomass reduction and changes in fermentation products. At the higher substrate concentration, substrate inhibition on biomass growth caused the decrease of carbohydrate degradation and hydrogen yield (based on hexose added). The ASBR showed higher biomass concentration and carbohydrate degradation efficiency than the CSTR, but hydrogen production in the ASBR was less effective than that in the CSTR at all the substrate concentrations.
Performance comparison of a continuous-flow stirred-tank reactor and an anaerobic sequencing batch reactor for fermentative hydrogen production depending on substrate concentration
S.-H. Kim, S.-K. Han, H.-S. Shin; Performance comparison of a continuous-flow stirred-tank reactor and an anaerobic sequencing batch reactor for fermentative hydrogen production depending on substrate concentration. Water Sci Technol 1 November 2005; 52 (10-11): 23–29. doi: https://doi.org/10.2166/wst.2005.0675
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