In-situ observations were performed at two different spatial and temporal scales, in order to get a better identification of the nature of the organic layer situated at the water–sediment interface, and which had previously been identified as major of combined sewer overflows organic loads. Its composition and its build up mechanisms during dry weather periods are presented. Results showed that the concept of dry weather accumulation and more generally the way organic sewer sediments are modelled needs to be reconsidered.

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