The objective of this study is to investigate the flow structure over biofilm experimentally and theoretically. Velocity and turbulence profiles over biofilm measured by a laser Doppler velocimetry were compared to simulated profiles using the k–ɛ turbulent model. Also, dissolved oxygen concentration profiles over biofilm are measured using a micro DO sensor. The k–ɛ turbulence model was proved to be a useful tool for the understanding of mass transfer inside and outside biofilm. Dissolved oxygen concentration profile inside and outside biofilm showed the existence of turbulent diffusion inside biofilm.

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