A new 16S rRNA-targeted oligonucleotide probe, specific for the cluster of fatty acid β-oxidizing syntrophic bacteria of the family Syntrophomonadaceae was designed for fluorescence in situ hybridization. This probe was evaluated with target as well as non-target cultures. Moreover this probe was assessed with butyrate and oleate degrading enrichment cultures and methanogenic sludges from full-scale plants. The results showed that the probe revealed the presence of fatty acid β-oxidizing syntrophic bacteria in some of the samples analyzed. However, cell quantification was possible only in enrichment cultures and in a flocculent sludge from a reactor that treats lipid-rich wastewaters, but not in methanogenic granular sludges from upflow anaerobic sludge blanket reactors.
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Research Article|
July 01 2006
Detection of fatty acid beta-oxidizing syntrophic bacteria by fluorescence in situ hybridization
R.J. Menes;
1Cátedra de Microbiología, Facultad de Química y Facultad de Ciencias, UDELAR. Gral Flores 2124, CC 1157, Montevideo, Uruguay
E-mail: jmenes@fq.edu.uy
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D. Travers
D. Travers
1Cátedra de Microbiología, Facultad de Química y Facultad de Ciencias, UDELAR. Gral Flores 2124, CC 1157, Montevideo, Uruguay
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Water Sci Technol (2006) 54 (2): 33–39.
Citation
R.J. Menes, D. Travers; Detection of fatty acid beta-oxidizing syntrophic bacteria by fluorescence in situ hybridization. Water Sci Technol 1 July 2006; 54 (2): 33–39. doi: https://doi.org/10.2166/wst.2006.483
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