A new 16S rRNA-targeted oligonucleotide probe, specific for the cluster of fatty acid β-oxidizing syntrophic bacteria of the family Syntrophomonadaceae was designed for fluorescence in situ hybridization. This probe was evaluated with target as well as non-target cultures. Moreover this probe was assessed with butyrate and oleate degrading enrichment cultures and methanogenic sludges from full-scale plants. The results showed that the probe revealed the presence of fatty acid β-oxidizing syntrophic bacteria in some of the samples analyzed. However, cell quantification was possible only in enrichment cultures and in a flocculent sludge from a reactor that treats lipid-rich wastewaters, but not in methanogenic granular sludges from upflow anaerobic sludge blanket reactors.
Skip Nav Destination
Research Article| July 01 2006
Detection of fatty acid beta-oxidizing syntrophic bacteria by fluorescence in situ hybridization
Water Sci Technol (2006) 54 (2): 33–39.
R.J. Menes, D. Travers; Detection of fatty acid beta-oxidizing syntrophic bacteria by fluorescence in situ hybridization. Water Sci Technol 1 July 2006; 54 (2): 33–39. doi: https://doi.org/10.2166/wst.2006.483
Download citation file: