The study was designed to genotype individual Cryptosporidium oocysts using an 18S rRNA gene-based semi-nested PCR and direct sequencing procedure. Positive PCR amplification was observed in all single C. parvum HNJ-1 oocyst samples tested. Semi-nested PCR and direct sequencing was applied to Cryptosporidium oocysts isolated from sewage and river water. The procedure could genotype 54% of FITC-stained single oocysts isolated from sewage and 32% from river water. The predominant genotype in both sewage and river water was C. parvum genotype 1, accounting for 33 and 25%, respectively, of all the FITC-stained intact Cryptosporidium oocysts present.
Genotyping of single Cryptosporidium oocysts isolated from sewage and river water
T. Hirata, A. Hashimoto; Genotyping of single Cryptosporidium oocysts isolated from sewage and river water. Water Sci Technol 1 August 2006; 54 (3): 197–202. doi: https://doi.org/10.2166/wst.2006.469
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