The monitoring of biofilm development at a small-scale is often observed to be a stochastic process. This raises important issues concerning the reproducibility of biofilm growth monitoring experiments. By realising that there are limits to the latter, a model of biofilm accumulation curves that takes into account the dynamics of seemingly random fluctuations resulting from sloughing events is proposed. The model is derived from a stochastic differential equation (SDE) based on the logistic equation, adding a stochastic term for the sloughing events and measurement noise. Experimental light absorbance data that correlate with biofilm biomass obtained from the development of phototrophic biofilms are analysed to illustrate the use of SDE modeling.

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