Radiotracer incubation experiments and beta microimaging, along with fluorescent in situ hybridisation (FISH), are proposed as a complementary approach to specific methanogenic activity testing and measurement of in vitro substrate utilisation rates to understand better the ecophysiology of anaerobic granular biofilms from wastewater treatment reactors.
Determination and localisation of in situ substrate uptake by anaerobic wastewater treatment granular biofilms
G. Collins, T. Mahony, A.-M. Enright, A. Gieseke, D. de Beer, V. O'Flaherty; Determination and localisation of in situ substrate uptake by anaerobic wastewater treatment granular biofilms. Water Sci Technol 1 April 2007; 55 (8-9): 369–376. doi: https://doi.org/10.2166/wst.2007.279
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