Wastewater treatment relies on careful integration of environmental engineering with microbial ecology. This would seem to be particularly the case when attempting to enhance survivability of organisms introduced from outside the main-stream reactor, i.e. bioaugmentation. Molecular biology tools were utilised in this study to assist in understanding the mechanisms of successful bioaugmentation. Molecular fingerprinting showed that side-stream reactor configuration strongly influenced ammonia-oxidising bacteria (AOB) community structure. In both lab-scale and full-scale systems, AOB communities in the side-stream and main-stream were very similar. The experimental systems revealed that a PFR side-stream produced greater diversity of AOB than a CSTR side-stream in a PFR main-stream system, whereas the full-scale side-stream resulted in essentially an AOB monoculture. Phylogenetic analysis revealed less diversity than molecular fingerprinting perhaps due to biases in the cloning/transformation procedure.
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Research Article|
June 01 2008
Ecological engineering of bioaugmentation from side-stream nitrification
R. C. Smith;
1Malcolm Pirnie Inc., 1900 Polaris Parkway, Columbus, OH 43240, USA E-mail: rocsmith@pirnie.com
E-mail: rocsmith@pirnie.com
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P. E. Saikaly;
P. E. Saikaly
2North Carolina St. University, Raleigh, NC 27695, USA E-mail: pesaikal@ncsu.edu
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K. Zhang;
K. Zhang
3Jones Edmunds, 730 NE Waldo Road, Gainesville, FL 32641, USA E-mail: kzhang@jonesedmunds.com
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S. Thomatos;
S. Thomatos
4The City College of New York, New York, NY 10031, USA E-mail: stefan.thomatos@va.gov
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D. B. Oerther
D. B. Oerther
5University of Cincinnati, Cincinnati, OH 45221, USA E-mail: daniel.oerther@uc.edu
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Water Sci Technol (2008) 57 (12): 1927–1933.
Citation
R. C. Smith, P. E. Saikaly, K. Zhang, S. Thomatos, D. B. Oerther; Ecological engineering of bioaugmentation from side-stream nitrification. Water Sci Technol 1 June 2008; 57 (12): 1927–1933. doi: https://doi.org/10.2166/wst.2008.628
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