Horseradish peroxidase (HRP) has the advantages of low cost, obtaining convenience, broad substrate specificity, etc, so it may be a suitable kind of enzyme to remove dilute emulsified oil and dissolved oil from water. The effect factors of the oil removal using HRP, such as pH, concentrations of HRP and H2O2, additives, Fe2 +  ion, temperature and reaction time were researched. When the initial oil concentration was 120 mg/L, the remaining oil concentrations of the synthetic oily water and an actual oily wastewater reached 24.83 mg/L and 21.30 mg/L, respectively. The treatment of oily water using HRP and H2O2 is feasible. It has good application prospects.

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