The treatment of high-saline wastewater from some salt-end markets including agro-food industry is a serious problem yet to be solved in some coastal cities. The conventional physical–chemical techniques are energy-consuming and their startup and running costs are still high. Biological methods using salt-tolerant bacterial strains for the treatment of hypersaline wastewater provide one possible solution. In this study, one salt-tolerant mutant named YWL-01 was screened out by sewage treatment and proved to be a genetically stable salt-tolerant strain for saline wastewater treatment. First, combined mutagenesis was done on an isolated sewage treatment strain Bacillus Y for the screening of salt tolerance, and 11 mutants were obtained after subculture for many times. Then, a secondary screening test was performed for COD (chemical oxygen demand) and TOC (total organic carbon) removal efficiency analyses. At last, the best mutant YWL-01 with increased capacity to treat saline wastewater was chosen for use. RAPD (Random Amplified Polymorphic DNA) analysis of genetic stability on the mutant YWL-01 showed that it is a hereditary mutant for the treatment of high-saline wastewater.
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Research Article|
December 01 2009
A novel salt-tolerant mutant YWL-01 for the treatment of saline wastewater
J. Liu;
J. Liu
1College of Life Science, Liaoning Normal University, Huanghe Road 850#, Dalian 116029, China E-mail: juaner-1983@hotmail.com
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G. L. Wang
1College of Life Science, Liaoning Normal University, Huanghe Road 850#, Dalian 116029, China E-mail: juaner-1983@hotmail.com
E-mail: guanlinwang@163.com
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Water Sci Technol (2009) 60 (11): 2869–2877.
Citation
J. Liu, G. L. Wang; A novel salt-tolerant mutant YWL-01 for the treatment of saline wastewater. Water Sci Technol 1 December 2009; 60 (11): 2869–2877. doi: https://doi.org/10.2166/wst.2009.750
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