Rotaviruses A (RV-A) infection is the most common cause of acute diarrheal diseases in infants and the dissemination of these viruses in the environment represents a public health hazard. The present study aims to evaluate reverse transcription-polymerase chain reaction (RT-PCR) based protocols for the detection of RV-A genes in different types of environmental samples. RV-A were concentrated by the adsorption-elution method using negatively charged membranes associated with a Centriprep Concentrator 50. The RV-A VP4, VP7 and VP6 genes were detected using RT-PCR in river water from the Amazon Hydrographic basin (Northern region) and from wastewater in a sewage treatment plant in Rio de Janeiro (Southeast region), Brazil. RV-A were successfully detected in water environmental samples by the methods used. The detection of the VP6 gene by RT-PCR was the most sensitive for detecting RV-A in environmental samples (44.0%), when compared to the detection of the VP4 (33.3%) and VP7 (25.3%) genes. Based on nucleotide sequence and phylogenetic analysis of the partial VP6 gene, 22 environmental samples were determined to be subgroup II (Wa-like). These results indicate that analysis of environmental samples could possibly make a valuable contribution to studies on the epidemiology of RV-A.
Environmental dissemination of group A rotavirus: P-type, G-type and subgroup characterization
F. F. M. Ferreira, F. R. Guimarães, T. M. Fumian, M. Victoria, C. B. Vieira, S. Luz, T. Shubo, J. P. G. Leite, M. P. Miagostovich; Environmental dissemination of group A rotavirus: P-type, G-type and subgroup characterization. Water Sci Technol 1 July 2009; 60 (3): 633–642. doi: https://doi.org/10.2166/wst.2009.413
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