A comparison of four different established models along with parameter estimation was carried out in order to explain the aerobic biodegradation of acetate in an activated sludge system. These models were investigated using experimental OUR data from batch experiments of three different concentration studies. Model calibration reveals that ASM1 model is not suitable to explain the observed experimental OUR during the famine phase implying storage compounds could play an important role during that stage. Besides, the model corresponds to the accumulation concept and is not well fitted for all concentrations studies though it includes the storage phenomena. Both the ASM3 model and the model for simultaneous storage and growth on substrate can well describe the acetate biodegradation process, however the OUR data alone is not sufficient to justify the suitability of those models. Simulated profiles using the model outputs demonstrate that storage is overestimated while ammonia degradation is underestimated in ASM3 compared to simultaneous growth and storage model. The current study also gives reasonable outcomes related to parameter estimation as compared with previous study which is statistically interpreted in this paper.

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