Anaerobic ammonium oxidation (anammox) is a novel nitrogen pathway catalyzed by anammox bacteria which are obligate anaerobic chemoautotrophs. In this study, enrichment culture of marine anammox bacteria (MAAOB) from the samples related to seawater was conducted. Simultaneous removal of ammonium and nitrite was confirmed in continuous culture inoculated with sediment of a sea-based waste disposal site within 50 days. However, no simultaneous nitrogen removal was observed in cultures inoculated with seawater-acclimated denitrifying sludge or with muddy sediment of tideland even during 200 days. Nitrogen removal rate of 0.13 kg/m3/day was achieved at nitrogen loading rate of 0.16 kg/m3/day after 320th days in the culture inoculated with the sediment of waste disposal site. The nitrogen removal ratio between ammonium nitrogen and nitrite nitrogen was 1:1.07. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that an abundance of the bacteria close to MAAOB and coexistence of ammonium oxidizing bacteria and denitrifying bacteria in the culture.
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Research Article|
January 01 2010
Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study
Y. Kawagoshi;
1Graduate School of Science & Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan E-mail: goshi@kumamoto-u.ac.jp; fuji_ko_com@yahoo.co.jp; k-furu@kumamoto-u.ac.jp; f-aya@tech.eng.kumamoto-u.ac.jp
E-mail: goshi@kumamoto-u.ac.jp
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Y. Nakamura;
Y. Nakamura
2Meidensha Corporation, 2-1-1 Osaki Shinagawa-ku, Tokyo 141-6029, Japan E-mail: nakamura-ya@mb.meidensha.co.jp
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H. Kawashima;
H. Kawashima
3Hanshin Engineering Co. Ltd., 2-26-7 Shikanjima Konohana-ku, Osaka 554-0014, Japan E-mail: kawashima@hanshin-pm.co.jp
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K. Fujisaki;
K. Fujisaki
1Graduate School of Science & Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan E-mail: goshi@kumamoto-u.ac.jp; fuji_ko_com@yahoo.co.jp; k-furu@kumamoto-u.ac.jp; f-aya@tech.eng.kumamoto-u.ac.jp
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K. Furukawa;
K. Furukawa
1Graduate School of Science & Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan E-mail: goshi@kumamoto-u.ac.jp; fuji_ko_com@yahoo.co.jp; k-furu@kumamoto-u.ac.jp; f-aya@tech.eng.kumamoto-u.ac.jp
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A. Fujimoto
A. Fujimoto
1Graduate School of Science & Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan E-mail: goshi@kumamoto-u.ac.jp; fuji_ko_com@yahoo.co.jp; k-furu@kumamoto-u.ac.jp; f-aya@tech.eng.kumamoto-u.ac.jp
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Water Sci Technol (2010) 61 (1): 119–126.
Citation
Y. Kawagoshi, Y. Nakamura, H. Kawashima, K. Fujisaki, K. Furukawa, A. Fujimoto; Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study. Water Sci Technol 1 January 2010; 61 (1): 119–126. doi: https://doi.org/10.2166/wst.2010.796
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