Anaerobic digestion of swine manure is carried out by a consortium of microbial species, including volatile fatty acid (VFA) producers, VFA-degraders and methanogens. The distribution of five phylogenetic groups within a plug-flow-type anaerobic bioreactor consisting of eight serially-connected tanks was examined through the sequential digestion of swine manure. Quantification was carried out using reverse transcription real-time PCR (RT-Q-PCR) assays targeting the 16S rRNA of Clostridium (cluster XIVa), Peptostreptococcus, Syntrophomonas, Methanosaeta, and Methanosarcina spp. The VFA producers Peptostreptococcus spp. and Clostridium spp. were found predominantly in compartments where hydrolysis/acidogenesis took place. The spatial distribution of the aceticlastic methanogens, Methanosaeta and Methanosarcina, within the bioreactor was not correlated with methanogenic activity. In contrast the VFA-degrading genus Syntrophomonas spp. was more abundant in compartments with elevated methanogenic activity. Multivariate statistical analyses of the RT-Q-PCR data have provided new insights into our understanding of how the various trophic groups were distributed within this bioreactor system. While the distribution of clostridia, peptostreptococci and Syntrophomonas corresponded to their known metabolic functions, aceticlastic methanogens were not apparently linked to the methanogenesis stage occurring in latter compartments, suggesting that hydrogenotrophic methanogens were the primary methane generators in this bioreactor. However, aceticlastic methanogens could be involved in compartments related to the hydrolysis/acidogenesis stage.
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Research Article|
March 01 2010
Spatial distribution of some microbial trophic groups in a plug-flow-type anaerobic bioreactor treating swine manure
Guylaine Talbot;
1Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 90, 2000 College Street, Lennoxville station, Sherbrooke QC, J1M 1Z3, Canada
E-mail: Guylaine.Talbot@AGR.GC.CA
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Caroline S. Roy;
Caroline S. Roy
1Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 90, 2000 College Street, Lennoxville station, Sherbrooke QC, J1M 1Z3, Canada
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Edward Topp;
Edward Topp
2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London ON, N5V 4T3, Canada
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Martin L. Kalmokoff;
Martin L. Kalmokoff
3Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food Canada, Kentville NS, B4N 1J5, Canada
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Stephen P. J. Brooks;
Stephen P. J. Brooks
4Health Canada, Bureau of Nutritional Sciences, Health Products and Foods Branch, Banting Research Centre, Ottawa ON, K1A 0L2, Canada
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Carole Beaulieu;
Carole Beaulieu
5Département de biologie, Université de Sherbrooke, Sherbrooke QC, J1K 2R1, Canada
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Marie-France Palin;
Marie-France Palin
1Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 90, 2000 College Street, Lennoxville station, Sherbrooke QC, J1M 1Z3, Canada
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Daniel I. Massé
Daniel I. Massé
1Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 90, 2000 College Street, Lennoxville station, Sherbrooke QC, J1M 1Z3, Canada
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Water Sci Technol (2010) 61 (5): 1147–1155.
Citation
Guylaine Talbot, Caroline S. Roy, Edward Topp, Martin L. Kalmokoff, Stephen P. J. Brooks, Carole Beaulieu, Marie-France Palin, Daniel I. Massé; Spatial distribution of some microbial trophic groups in a plug-flow-type anaerobic bioreactor treating swine manure. Water Sci Technol 1 March 2010; 61 (5): 1147–1155. doi: https://doi.org/10.2166/wst.2010.014
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