With the aid of analysis software (Minitab 14.0), the formulation of pure culture in Mineral Salts Medium (MSM) can be optimized for several responses and the best formulation can be obtained. The influence of the different mixtures of three strains in the pure culture in MSM on the flavor components in decolorization of Methyl Red (with initial total cell density fixed at OD600 = 1 and in addition of 750 ppm of dye) was studied using equilateral triangle diagram and mixture experimental design to assess color and COD removal during species evolution. The regression model on microorganism composition and main metabolites was established. The results suggested that the highest predictable specific decolorization rate and Chemical Oxygen Demand (COD) were 77.97 and 93.77%. Based on these, the response values that satisfied all expectations were optimized, and the optimal composition of the mixed consortium for the dedolorization and COD removal were (Sphingomonas paucimobilis 45.20%, Bacillus sp 61.94% and Staphylococcus epidermidis 80.00%) and (Sphingomonas paucimobilis 77.03%, Bacillus sp 86.42% and Staphylococcus epidermidis 71.74%) respectively. Very high regression coefficient between the variables and the responses: decolorization and COD removal were respectively R2=0.96 and 0.81 indicated excellent evaluation of experimental data by polynomial regression model.
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Research Article|
December 01 2010
Application of the mixture design to optimise the formulation of active consortia to decolorize azo-dye methyl red Available to Purchase
Lamia Ayed;
1Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne, 5000 Monastir, Tunisie
E-mail: [email protected]
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Besma Harbi;
Besma Harbi
1Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne, 5000 Monastir, Tunisie
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Abdelkrim Cheref;
Abdelkrim Cheref
2Laboratoire de Géochimie et Physicochimie de l'Eau, CERTE, Technopole Borj Cedria, 2073 Nabeul, Tunisie
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Amina Bakhrouf;
Amina Bakhrouf
1Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne, 5000 Monastir, Tunisie
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Sami Achour
Sami Achour
3Unité de recherche Génome Humain, Diagnostic Immunitaire et Valorisation à l'Institut Supérieur de Biotechnologie, 5000 Monastir, Tunisie
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Water Sci Technol (2010) 62 (12): 2837–2845.
Citation
Lamia Ayed, Besma Harbi, Abdelkrim Cheref, Amina Bakhrouf, Sami Achour; Application of the mixture design to optimise the formulation of active consortia to decolorize azo-dye methyl red. Water Sci Technol 1 December 2010; 62 (12): 2837–2845. doi: https://doi.org/10.2166/wst.2010.709
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