This study investigates microbial population dynamics in granular sequencing batch reactors (GSBR). The experimental results of DGGE fingerprint of sludge demonstrated that the microbial community structure of sludge shifted significantly during granulation period and nutrient removal improvement period. After reactor performance and physical characteristics of sludge reached steady state, microbial population of sludge became relatively stable. The high similarity of microbial community structure between co-existed flocculated sludge and granular sludge in GSBR at different operation phases indicated that similar microbial consortium could exist in compact aggregated form or in amorphous flocculated form. Therefore, strong selection pressure was still required to wash out flocs to maintain the stability of reactor operation. In addition, it was found that substrate type had considerable impact on microbial species selection and enrichment in granular sludge. The clone library of granular sludge showed that microbial species in divisions of α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria and Bacteroidetes existed within acetate-fed granule communities and Thauera spp. from β-Proteobacteria accounted for 49% of the total clones in the whole clone library. It is thus speculated that Thauera spp. are important for the formation of acetate-fed granules under the conditions used in this study, maintaining the integrity of granules or substrate degradation.
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Research Article|
September 01 2010
Microbial population dynamics of granular aerobic sequencing batch reactors during start-up and steady state periods
Y. Q. Liu;
1Institute of Environmental Science and Engineering, Nanyang Technological University, 18, Nanyang Drive, Singapore 637723, Singapore E-mail: cyqliu@ntu.edu.sg; yhkong@ntu.edu.sg; zhangxing@ntu.edu.sg; wongfs@ntu.edu.sg
E-mail: cyqliu@ntu.edu.sg
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Y. H. Kong;
Y. H. Kong
1Institute of Environmental Science and Engineering, Nanyang Technological University, 18, Nanyang Drive, Singapore 637723, Singapore E-mail: cyqliu@ntu.edu.sg; yhkong@ntu.edu.sg; zhangxing@ntu.edu.sg; wongfs@ntu.edu.sg
2School of Environment, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China
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R. Zhang;
R. Zhang
3Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, Engineering Drive 3, Singapore 117576, Singapore E-mail: esezr@nus.edu.sg
6State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
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X. Zhang;
X. Zhang
1Institute of Environmental Science and Engineering, Nanyang Technological University, 18, Nanyang Drive, Singapore 637723, Singapore E-mail: cyqliu@ntu.edu.sg; yhkong@ntu.edu.sg; zhangxing@ntu.edu.sg; wongfs@ntu.edu.sg
7College of Architecture and Environment, Sichuan University, Chengdu 610065, China E-mail: wenjujiang@scu.edu.cn
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F. S. Wong;
F. S. Wong
1Institute of Environmental Science and Engineering, Nanyang Technological University, 18, Nanyang Drive, Singapore 637723, Singapore E-mail: cyqliu@ntu.edu.sg; yhkong@ntu.edu.sg; zhangxing@ntu.edu.sg; wongfs@ntu.edu.sg
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J. H. Tay;
J. H. Tay
4School of Civil and Environmental Engineering, Nanyang Technological University, 50, Nanyang Avenue, Singapore 639798, Singapore E-mail: cjhtay@ntu.edu.sg
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J. R. Zhu;
J. R. Zhu
6State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
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W. J. Jiang;
W. J. Jiang
7College of Architecture and Environment, Sichuan University, Chengdu 610065, China E-mail: wenjujiang@scu.edu.cn
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W. T. Liu
W. T. Liu
3Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, Engineering Drive 3, Singapore 117576, Singapore E-mail: esezr@nus.edu.sg
5Department of Civil and Environmental Engineering, University of Illinois-Urbana Champaign, Urbana Illinois 61801, USA E-mail: wentso.liu@gmail.com
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Water Sci Technol (2010) 62 (6): 1281–1287.
Citation
Y. Q. Liu, Y. H. Kong, R. Zhang, X. Zhang, F. S. Wong, J. H. Tay, J. R. Zhu, W. J. Jiang, W. T. Liu; Microbial population dynamics of granular aerobic sequencing batch reactors during start-up and steady state periods. Water Sci Technol 1 September 2010; 62 (6): 1281–1287. doi: https://doi.org/10.2166/wst.2010.408
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