In this study, an environmental sampling campaign was conducted to detect internalized E. coli and C. jejuni bacteria in zooplankton and amoebae samples collected at various stages of three water treatment plants in Amsterdam, the Netherlands. Eight sampling locations were selected and sampling was performed twice, at a two-week interval, at each location. Chlorination was used to inactivate free (external) bacteria in the concentrated zooplankton samples and sonication was used to disrupt zooplankton organisms in order to release and recover internalized bacteria. Zooplankton enumeration was performed by microscopy. No internalized E. coli or C. jejuni bacteria were recovered from all of the samples analyzed. The occurrence of internalized E. coli or C. jejuni bacteria in drinking water was estimated to be lower than one internalized bacteria in 105 zooplankton organisms, as derived from the detection limit of the sampling campaign. By using the QMRA approach and the Beta-Poisson model, a risk of infection of less than 9.2E-6 and 5.9E-5 was estimated for internalized E. coli and C. jejuni in drinking water, respectively. This study remains preliminary due to the limited number of samples taken at each location.
Skip Nav Destination
Article navigation
Research Article|
January 01 2011
Preliminary study on the occurrence and risk arising from bacteria internalized in zooplankton in drinking water
F. Bichai;
1NSERC Industrial Chair on Drinking Water, École Polytechnique de Montreal, Department of Civil, Geological, and Mining Engineering, P.O. Box 6079, Downtown Station, Montreal (Quebec) Canada H3C 3A7 E-mail: [email protected]
E-mail: [email protected]
Search for other works by this author on:
W. Hijnen;
W. Hijnen
2KWR Watercycle Research Institute, Postbus 1072, 3430 BB Nieuwegein, The Netherlands E-mail: [email protected]
Search for other works by this author on:
Eric Baars;
Eric Baars
3Waternet, Provincialeweg 21, 1108 AA Amsterdam, The Netherlands E-mail: [email protected]
Search for other works by this author on:
M. Rosielle;
M. Rosielle
4Het Waterlaboratorium, J.W. Lucasweg 2, 2031 BE Haarlem, Postbus 734, 2003 RS Haarlem, The Netherlands E-mail: [email protected]
Search for other works by this author on:
Y. Dullemont;
Y. Dullemont
3Waternet, Provincialeweg 21, 1108 AA Amsterdam, The Netherlands E-mail: [email protected]
Search for other works by this author on:
B. Barbeau
B. Barbeau
1NSERC Industrial Chair on Drinking Water, École Polytechnique de Montreal, Department of Civil, Geological, and Mining Engineering, P.O. Box 6079, Downtown Station, Montreal (Quebec) Canada H3C 3A7 E-mail: [email protected]
Search for other works by this author on:
Water Sci Technol (2011) 63 (1): 108–114.
Citation
F. Bichai, W. Hijnen, Eric Baars, M. Rosielle, Y. Dullemont, B. Barbeau; Preliminary study on the occurrence and risk arising from bacteria internalized in zooplankton in drinking water. Water Sci Technol 1 January 2011; 63 (1): 108–114. doi: https://doi.org/10.2166/wst.2011.018
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
eBook
Pay-Per-View Access
$38.00