The goal of this study is to evaluate the feasibility of PHA biosynthesis from kraft mill effluent using the batch system evaluating the biomass origin and C:N relationship influence. To evaluate feasibility, batch assays were carried out. Also, two levels of the BOD5:N:P relationship (100:5:1 and 100:1:0.2) and three different sludge origins were considered. Inocula were obtained from activated sludge treatment plants for a) sewage (SAS), b) paper mill (PAS) and c) kraft (KAS). The results show that the maximum Biological Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) removal was 80.5% and 59.7% respectively using KAS as inoculum. In these assays, kinetics constants were 17.9±3.2 mg L−1 and 46.5±1.2 d−1 for (KCOD) and (rmax), respectively under a BOD5:N:P relationship of 100:5:1. The maximum PHA accumulation was obtained under a BOD5:N:P relationship of 100:1:0.2 on the third day of batch assays using PAS sludge with 25.72% of the cells accumulating PHA and on the fifth day in batch using SAS and KAS sludge with 25.85% and 30.40% of cells accumulating PHA, respectively. Yields obtained for the 100:1:0.2 relationships ranged from 0.10–0.14 mg PHA mg−1 COD.
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Research Article|
February 01 2011
Polyhydroxyalkanoates (PHA) biosynthesis from kraft mill wastewaters: biomass origin and C:N relationship influence
G. Pozo;
G. Pozo
1Environmental Sciences Center EULA-Chile, Universidad de Concepción, P. O. Box 160-C, Concepción-Chile
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A. C. Villamar;
A. C. Villamar
1Environmental Sciences Center EULA-Chile, Universidad de Concepción, P. O. Box 160-C, Concepción-Chile
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M. Martínez;
M. Martínez
2Microbiology Department, University of ConcepciónP.O. Box 160-C, Concepción, Chile
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G. Vidal
1Environmental Sciences Center EULA-Chile, Universidad de Concepción, P. O. Box 160-C, Concepción-Chile
Tel.: 56-41-2204067 Fax:
56-41-2207076
; E-mail: glvidal@udec.cl
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Water Sci Technol (2011) 63 (3): 449–455.
Citation
G. Pozo, A. C. Villamar, M. Martínez, G. Vidal; Polyhydroxyalkanoates (PHA) biosynthesis from kraft mill wastewaters: biomass origin and C:N relationship influence. Water Sci Technol 1 February 2011; 63 (3): 449–455. doi: https://doi.org/10.2166/wst.2011.242
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