This study aimed to verify the biological anaerobic treatability of tebuconazole effluent manufacturing. For this purpose, two preliminary batch tests were performed using an ‘anaerobic respirometer’ adjusting the initial pH of the effluent at 7.0 and 8.0, respectively (first phase). In addition, two tests (second phase) were run using sequential batch anaerobic fermenters, the first operated at different hydraulic detention times (10 and 16.7 days) and the second with different initial dilutions of the effluent (5 and 20%). The chemical oxygen demand (COD) removal observed on the anaerobic respirometer tests was in the range of 66–81%. According to the preliminary batch tests an optimal value of initial COD concentration and amount of biomass was identified, which was considered for the fermenters start up. However, it was observed that the optimal relation provided by the respirometer test was not a good parameter of operational control for the fermenters due to the accumulation of inhibitory substances, which affected the microbial activity and took the system to collapse. The initial dilution of the effluent (5 and 20%) was essential for the stability of the anaerobic system, allowing COD removals above 74% during this study.

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