A rapid non-derivatisation gas chromatographic (GC) method for quantification of palmitic, stearic and oleic acids was achieved using a flame ionisation detector and a highly polar capillary column at elevated temperature. These long chain fatty acids (LCFA) can accumulate in anaerobic digesters and a simple extraction method was also developed to permit a more rapid sample turn-around time, facilitating more frequent monitoring. The GC method was satisfactory in terms of peak separation, signal response, reproducibility and linearity range. The extraction method achieved recoveries of 103.8, 127.2 and 84.2% for palmitic, stearic and oleic acid respectively. The method was tested on digestate from mesophilic laboratory-scale digesters fed with source-segregated domestic food waste, and showed good repeatability between replicate samples. It was observed that the concentrations of stearic and palmitic acid in digesters routinely supplemented with trace elements were lower in proportion to the applied lipid loading than those without supplementation.
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Research Article|
August 01 2012
Determination of long chain fatty acids in anaerobic digesters using a rapid non-derivatisation GC-FID method Available to Purchase
Ying Jiang;
1Faculty of Engineering and the Environment, University of Southampton, Southampton SO17 1BJ, UK
E-mail: [email protected]
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Yue Zhang;
Yue Zhang
1Faculty of Engineering and the Environment, University of Southampton, Southampton SO17 1BJ, UK
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Charles J. Banks
Charles J. Banks
1Faculty of Engineering and the Environment, University of Southampton, Southampton SO17 1BJ, UK
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Water Sci Technol (2012) 66 (4): 741–747.
Article history
Received:
June 16 2011
Accepted:
January 04 2012
Citation
Ying Jiang, Yue Zhang, Charles J. Banks; Determination of long chain fatty acids in anaerobic digesters using a rapid non-derivatisation GC-FID method. Water Sci Technol 1 August 2012; 66 (4): 741–747. doi: https://doi.org/10.2166/wst.2012.063
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