Azo dyes are extensively used, but are recalcitrant and refractory. In this study, an indigenous strain DH-6 was isolated and identified as Aeromonas sp. based on 16S rDNA analysis for its excellent methyl orange (MO) decolorizing capability. Plackett–Burman design and response surface methodology (RSM) were employed to investigate the effect of operational parameters on decolorization and to optimize the decolorization process. Based on the results the concentrations of glucose, Na2HPO4 and MO and temperature were selected as the four significant parameters of RSM. The optimal conditions for MO decolorization by the strain were as follows: 3.0 g/L glucose, 4.9 g/L Na2HPO4, 100 mg/L MO, and at 40 °C. The verification tests showed that 95.5% decolorization was observed after incubation for 2 h, which is within the confidence interval. Under the optimal conditions, the kinetics of the decolorization fitted the first-order model well (R2 = 0.969). As the strain DH-6 still showed a good decolorizing capability at a relatively high temperature, it is considered a candidate for azo dye bioremediation in some tropical or subtropical regions.
Skip Nav Destination
Article navigation
Research Article|
February 28 2014
Rapid decolorization of methyl orange by a novel Aeromonas sp. strain DH-6
Lin-Na Du;
Lin-Na Du
1Department of Agriculture and Biotechnology, Wenzhou Key Laboratory for Quality and Safty of Agriculture products, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang Province, China
Search for other works by this author on:
Gang Li;
Gang Li
1Department of Agriculture and Biotechnology, Wenzhou Key Laboratory for Quality and Safty of Agriculture products, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang Province, China
Search for other works by this author on:
Fang-Cheng Xu;
Fang-Cheng Xu
1Department of Agriculture and Biotechnology, Wenzhou Key Laboratory for Quality and Safty of Agriculture products, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang Province, China
Search for other works by this author on:
Xiu Pan;
Xiu Pan
1Department of Agriculture and Biotechnology, Wenzhou Key Laboratory for Quality and Safty of Agriculture products, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang Province, China
Search for other works by this author on:
Ling-Ning Wen;
Ling-Ning Wen
1Department of Agriculture and Biotechnology, Wenzhou Key Laboratory for Quality and Safty of Agriculture products, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang Province, China
Search for other works by this author on:
Yang Wang
1Department of Agriculture and Biotechnology, Wenzhou Key Laboratory for Quality and Safty of Agriculture products, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang Province, China
E-mail: [email protected]
Search for other works by this author on:
Water Sci Technol (2014) 69 (10): 2004–2013.
Article history
Received:
June 19 2013
Accepted:
February 13 2014
Citation
Lin-Na Du, Gang Li, Fang-Cheng Xu, Xiu Pan, Ling-Ning Wen, Yang Wang; Rapid decolorization of methyl orange by a novel Aeromonas sp. strain DH-6. Water Sci Technol 1 May 2014; 69 (10): 2004–2013. doi: https://doi.org/10.2166/wst.2014.099
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
eBook
Pay-Per-View Access
$38.00