A two-phase system for biogas production from cheese whey wastewater (CWW) was designed, set up and operated at laboratory and full scale for a whole cheese production season (8–9 months). The high efficiency and stability of the laboratory-scale system was demonstrated under various organic loading rates (OLRs) reaching 13 g chemical oxygen demand (COD) L−1d−1 and producing up to 9 L L−1d−1 of biogas (approximately 55% in methane). The COD removal was above 95% and the pH was maintained above 6.3 without any chemical addition. The full-scale system was operated at lower OLRs than its normal capacity, following the good response and high stability in disturbances of the laboratory-scale unit.

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