Isolating new diesel-oil-degrading microorganisms from crude-oil contaminated sites and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude-oil exploitation. In this research, new hydrocarbon-degrading bacteria and fungi were isolated from the crude-oil contaminated soil of the oil-fields in the Amazon rainforest of north-east Ecuador by using a soil enrichment technique. Degradation analysis was tracked by gas chromatography and a flame ionization detector. Under laboratory conditions, maximum degradability of the total n-alkanes reached up to 77.34 and 62.62 removal ratios after 30 days of incubation for the evaporated diesel oil by fungi (isolate-1) and bacteria (isolate-1), respectively. The 16S/18S rDNA sequence analysis indicated that the microorganisms were most closely (99–100%) related to Bacillus cereus (isolate-1), Bacillus thuringiensis (isolate-2), Geomyces pannorum (isolate-1), and Geomyces sp. (isolate-2). Therefore, these strains enable the degradation of hydrocarbons as the sole carbon source, and these findings will benefit these strains in the remediation of oil-polluted environments and oil exploitation.
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Research Article|
March 28 2015
Novel diesel-oil-degrading bacteria and fungi from the Ecuadorian Amazon rainforest
N. R. Maddela;
1Faculty of Environmental Engineering, Universidad Estatal Amazónica, Puyo, Ecuador
E-mail: mnraju8875@gmail.com
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M. Masabanda;
M. Masabanda
2Department of Life Sciences, Universidad de las Fuerzas Armadas, ESPE, Quito, Ecuador
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M. Leiva-Mora
M. Leiva-Mora
3Instituto de Biotecnología de las Plantas (IBP), Universidad Central Marta Abreu de Las Villas, Santa Clara, Cuba
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Water Sci Technol (2015) 71 (10): 1554–1561.
Article history
Received:
November 15 2014
Accepted:
March 13 2015
Citation
N. R. Maddela, M. Masabanda, M. Leiva-Mora; Novel diesel-oil-degrading bacteria and fungi from the Ecuadorian Amazon rainforest. Water Sci Technol 1 May 2015; 71 (10): 1554–1561. doi: https://doi.org/10.2166/wst.2015.142
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