Human adenovirus (HAdV) infections can occur throughout the year. Cases of HAdV-associated respiratory disease have been more common in the late winter, spring, and early summer. In this study, to provide viral pollution data for further epidemiological studies and governmental actions, the presence of HAdV in the aquatic environment was quantitatively surveyed in the summer. This study was conducted to compare the efficiencies of nested-PCR (polymerase chain reaction) and qPCR (quantitative PCR) for detecting HAdV in environmental waters. A total of 73 water samples were collected from Puzi River in Taiwan and subjected to virus concentration methods. In the results, qPCR had much better efficiency for specifying the pathogen in river sample. HAdV41 was detected most frequently in the river water sample (10.9%). The estimated HAdV concentrations ranged between 6.75 × 102 and 2.04 × 109 genome copies/L. Significant difference was also found in heterotrophic plate counts, conductivity, water temperature, and water turbidity between presence/absence of HAdV. HAdV in the Puzi River may pose a significant health risk.
Nested-PCR and TaqMan real-time quantitative PCR assays for human adenoviruses in environmental waters
Wen-Chien Huang, Yi-Pen Chou, Po-Min Kao, Tsui-Kang Hsu, Hung-Chang Su, Ying-Ning Ho, Yi-Chun Yang, Bing-Mu Hsu; Nested-PCR and TaqMan real-time quantitative PCR assays for human adenoviruses in environmental waters. Water Sci Technol 27 April 2016; 73 (8): 1832–1841. doi: https://doi.org/10.2166/wst.2016.004
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