Human adenovirus (HAdV) infections can occur throughout the year. Cases of HAdV-associated respiratory disease have been more common in the late winter, spring, and early summer. In this study, to provide viral pollution data for further epidemiological studies and governmental actions, the presence of HAdV in the aquatic environment was quantitatively surveyed in the summer. This study was conducted to compare the efficiencies of nested-PCR (polymerase chain reaction) and qPCR (quantitative PCR) for detecting HAdV in environmental waters. A total of 73 water samples were collected from Puzi River in Taiwan and subjected to virus concentration methods. In the results, qPCR had much better efficiency for specifying the pathogen in river sample. HAdV41 was detected most frequently in the river water sample (10.9%). The estimated HAdV concentrations ranged between 6.75 × 102 and 2.04 × 109 genome copies/L. Significant difference was also found in heterotrophic plate counts, conductivity, water temperature, and water turbidity between presence/absence of HAdV. HAdV in the Puzi River may pose a significant health risk.
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Research Article|
January 20 2016
Nested-PCR and TaqMan real-time quantitative PCR assays for human adenoviruses in environmental waters Available to Purchase
Wen-Chien Huang;
Wen-Chien Huang
1Department of Medicine, Mackay Medicine College, Taipei, Taiwan, ROC and Department of Thoracic Surgery, Mackay Memorial Hospital, Taipei, Taiwan, ROC
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Yi-Pen Chou;
Yi-Pen Chou
2Department of Anesthesiology, Cheng Hsin General Hospital, Taipei, Taiwan, ROC
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Po-Min Kao;
Po-Min Kao
3Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC
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Tsui-Kang Hsu;
Tsui-Kang Hsu
4Department of Ophthalmology, Cheng Hsin General Hospital, Taipei, Taiwan, ROC and Department of Life Science, Institute of Molecular Biology, Institute of Biomedical Science, National Chung Cheng University, Chiayi, Taiwan, ROC
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Hung-Chang Su;
Hung-Chang Su
2Department of Anesthesiology, Cheng Hsin General Hospital, Taipei, Taiwan, ROC
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Ying-Ning Ho;
Ying-Ning Ho
3Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC
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Yi-Chun Yang;
Yi-Chun Yang
3Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC
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Bing-Mu Hsu
3Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC
E-mail: [email protected]
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Water Sci Technol (2016) 73 (8): 1832–1841.
Article history
Received:
July 29 2015
Accepted:
December 22 2015
Citation
Wen-Chien Huang, Yi-Pen Chou, Po-Min Kao, Tsui-Kang Hsu, Hung-Chang Su, Ying-Ning Ho, Yi-Chun Yang, Bing-Mu Hsu; Nested-PCR and TaqMan real-time quantitative PCR assays for human adenoviruses in environmental waters. Water Sci Technol 27 April 2016; 73 (8): 1832–1841. doi: https://doi.org/10.2166/wst.2016.004
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