The aim of this study was to analyze the effect of the addition of rice straw and clay residuals on the prokaryote methane-producing community structure in a semi-continuously stirred tank reactor fed with swine manure. Molecular techniques, including terminal restriction fragment length polymorphism and a comparative nucleotide sequence analyses of the prokaryotic 16S rRNA genes, were performed. The results showed a positive effect of clay addition on methane yield during the co-digestion of swine manure and rice straw. At the digestion of swine manure, the bacterial phylum Firmicutes and the archaeal family Methanosarcinaceae, particularly Methanosarcina species, were predominant. During the co-digestion of swine manure and rice straw the microbial community changed, and with the addition of clay residual, the phylum Bacteroidetes predominated. The new nutritional conditions resulted in a shift in the archaeal family Methanosarcinaceae community as acetoclastic Methanosaeta species became dominant.
Prokaryote community dynamics in anaerobic co-digestion of swine manure, rice straw and industrial clay residuals
Janet Jiménez, Susanne Theuerl, Ingo Bergmann, Michael Klocke, Gilda Guerra, Osvaldo Romero-Romero; Prokaryote community dynamics in anaerobic co-digestion of swine manure, rice straw and industrial clay residuals. Water Sci Technol 17 August 2016; 74 (4): 824–835. doi: https://doi.org/10.2166/wst.2016.170
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