Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20–60 mg/L), inoculum size (4–8%) and temperature (25–35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10–30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.
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Research Article|
January 23 2017
Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441
Ganta Upendar;
Ganta Upendar
1Department of Chemical Engineering, National Institute of Technology Durgapur, Durgapur 713209, India
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Susmita Dutta;
1Department of Chemical Engineering, National Institute of Technology Durgapur, Durgapur 713209, India
E-mail: susmita_che@yahoo.com
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Pinaki Bhattacharya;
Pinaki Bhattacharya
2Department of Chemical Engineering, Heritage Institute of Technology Kolkata, Kolkata 700107, India
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Abhishek Dutta
Abhishek Dutta
3Departement Materiaalkunde, KU Leuven, KasteelparkArenberg 44 bus 2450, Heverlee-Leuven B-3001, Belgium
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Water Sci Technol (2017) 75 (7): 1572–1583.
Article history
Received:
September 16 2016
Accepted:
December 16 2016
Citation
Ganta Upendar, Susmita Dutta, Pinaki Bhattacharya, Abhishek Dutta; Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441. Water Sci Technol 12 April 2017; 75 (7): 1572–1583. doi: https://doi.org/10.2166/wst.2017.031
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