Biogenic manganese oxides (BioMnOx) can be applied for the effective removal and recovery of trace metals from wastewater because of their high adsorption capacity. Although a freshwater continuous-flow system for a nitrifier-based Mn-oxidizing microbial community for producing BioMnOx has been developed so far, a seawater continuous-flow bioreactor system for BioMnOx production has not been established. Here, we report BioMnOx production by a methanotroph-based microbial community by using a continuous-flow bioreactor system. The bioreactor system was operated using a deep-sea sediment sample as the inoculum with methane as the energy source for over 2 years. The BioMnOx production became evident after 370 days of reactor operation. The maximum Mn oxidation rate was 11.4 mg L−1 day−1. An X-ray diffraction analysis showed that the accumulated BioMnOx was birnessite. 16S rRNA gene-based clone analyses indicated that methanotrophic bacterial members were relatively abundant in the system; however, none of the known Mn-oxidizing bacteria were detected. A continuous-flow bioreactor system coupled with nitrification was also run in parallel for 636 days, but no BioMnOx production was observed in this bioreactor system. The comparative experiments indicated that the methanotroph-based microbial community, rather than the nitrifier-based community, was effective for BioMnOx production under the marine environmental conditions.
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Research Article|
June 19 2017
Biotic manganese oxidation coupled with methane oxidation using a continuous-flow bioreactor system under marine conditions
Shingo Kato;
Shingo Kato
1Ore Genesis Research Unit, Project Team for Development of New-generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2Research and Development Center for Submarine Resources, JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan
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Masayuki Miyazaki;
Masayuki Miyazaki
3Department of Subsurface Geobiological Analysis and Research (D-SUGAR), JAMSTEC, 2-15 Natsuhima-cho, Yokosuka, Kanagawa 237-0061, Japan
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Sakiko Kikuchi;
Sakiko Kikuchi
1Ore Genesis Research Unit, Project Team for Development of New-generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2Research and Development Center for Submarine Resources, JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan
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Teruhiko Kashiwabara;
Teruhiko Kashiwabara
1Ore Genesis Research Unit, Project Team for Development of New-generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2Research and Development Center for Submarine Resources, JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan
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Yumi Saito;
Yumi Saito
3Department of Subsurface Geobiological Analysis and Research (D-SUGAR), JAMSTEC, 2-15 Natsuhima-cho, Yokosuka, Kanagawa 237-0061, Japan
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Eiji Tasumi;
Eiji Tasumi
3Department of Subsurface Geobiological Analysis and Research (D-SUGAR), JAMSTEC, 2-15 Natsuhima-cho, Yokosuka, Kanagawa 237-0061, Japan
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Katsuhiko Suzuki;
Katsuhiko Suzuki
1Ore Genesis Research Unit, Project Team for Development of New-generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2Research and Development Center for Submarine Resources, JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan
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Ken Takai;
Ken Takai
1Ore Genesis Research Unit, Project Team for Development of New-generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2Research and Development Center for Submarine Resources, JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan
3Department of Subsurface Geobiological Analysis and Research (D-SUGAR), JAMSTEC, 2-15 Natsuhima-cho, Yokosuka, Kanagawa 237-0061, Japan
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Linh Thi Thuy Cao;
Linh Thi Thuy Cao
4Department of Civil and Environmental Engineering, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8511, Japan
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Akiyoshi Ohashi;
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Akiyoshi Ohashi
4Department of Civil and Environmental Engineering, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8511, Japan
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Hiroyuki Imachi
2Research and Development Center for Submarine Resources, JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan
3Department of Subsurface Geobiological Analysis and Research (D-SUGAR), JAMSTEC, 2-15 Natsuhima-cho, Yokosuka, Kanagawa 237-0061, Japan
E-mail: [email protected]
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Water Sci Technol (2017) 76 (7): 1781–1795.
Article history
Received:
December 27 2016
Accepted:
June 05 2017
Citation
Shingo Kato, Masayuki Miyazaki, Sakiko Kikuchi, Teruhiko Kashiwabara, Yumi Saito, Eiji Tasumi, Katsuhiko Suzuki, Ken Takai, Linh Thi Thuy Cao, Akiyoshi Ohashi, Hiroyuki Imachi; Biotic manganese oxidation coupled with methane oxidation using a continuous-flow bioreactor system under marine conditions. Water Sci Technol 9 October 2017; 76 (7): 1781–1795. doi: https://doi.org/10.2166/wst.2017.365
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