Wastewater treatment systems have great importance in dealing with increasing environmental pollution. In this study, a specially designed and constructed flow-through electrochemical cell was used to enhance the electrosorptive removal capacity of 2,4-Dichlorofenoxyacetic acid (2,4-D) pesticide from aqueous solutions onto high area activated carbon cloth (ACC) fixed-bed electrodes. The change in concentration of 2,4-D during the electrosorption process was followed by an online UV–Vis spectrophotometric system. Effects of operational parameters such as volumetric flow rate, applied potential and existence of Na2SO4 salt on electrosorption of 2,4-D were examined. Optimum values were found to be 10 mL·min−1 for volumetric flow rate and +900 mV for the applied potential to polarize ACC. Maximum capacity of 729 mg·gACC−1 has been achieved for removal of 2,4-D pesticides.