The purpose of this study is to explore the nitrogen removal efficiency of the Moving Bed Biofilm Reactor (MBBR) reactor under different ammonia nitrogen concentrations (states P1-P5), especially the composition of various forms of nitrogen, related genes and Microbial community structure and succession law in the effluent of the reactor Structure and succession law. The results show that the average efficiency of MBBR effluent denitrification is 63.63%.The concentration dynamics of NO3-N and NO2-N in the effluent indicated a relative short-range nitrification and denitrification reaction in MBBR.The results of 16SrRNA sequencing of P1-P5 microbial samples found that changes in the concentration of ammonia nitrogen in the influent produced significant changes in the composition of the microbial community in the MBBR. The genus Ottowia and Flavobacterium played an important role in the nitrogen removal of the MBBR system.

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