Two effects of Nocardia in waste activated sludge (WAS) were investigated: (i) the influence of WAS in the solids treatment recycle streams on Nocardia persistence in the activated sludge, and (ii) the effect of Nocardia in WAS on anaerobic digester foaming. About 4% of the Nocardia present in the mixed liquor was due to seeding from the WAS solids in the dissolved air flotation thickener recycle stream recycle. Nocardia filaments in WAS at levels of between 104 to 106 intersections/g VSS resulted in Nocardia levels of approximately 104 to 105 intersections/g VSS in anaerobic digesters that treated both WAS and primary sludge. The effect of disinfecting these Nocardia filaments in the WAS with Cl2 was investigated at Cl2 dose ranges of 20-60 mg Cl2/l WAS and 100-200 mg Cl2/l WAS on a lab scale using batch foaming tests to assess success. Chlorination with 20 - 60 mg Cl2/l WAS approximately doubled both sludge foaming potential and foam stability. At Cl2 doses of 100-200 mg Cl2/l WAS, foaming potential was increased almost 10-fold, and foam stability was increased by 2.5 times. These results indicate that chlorination of WAS feed to an anaerobic digester for inactivation of Nocardia should not be practiced.

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