The purpose of this study was to evaluate the efficiency and feasibility of treating brewery wastewater with an Anaerobic Fluidized Bed(AFB) reactor at ambient temperatures of about 25°C. Results from nearly one year of tests have demonstrated that the reactor has a volumetric loadings rate of 27-30kgCOD/m3.d with 2.5 h of hydraulic retention time (HRT). The COD removal rate can reach 85% or more under stable operating conditions. The average production rate of biogas was 0.45m3/kgCOD removed, of which the CH4 content was 72%. In addition, the formation mechanism of the biofilm was analyzed, the measures of rapid start up of the reactor was investigated. The operational characteristics of the AFB reactor were also discussed.