The impact of river regulation has been to reduce flood frequencies on the Murray floodplain below Mildura. The lower frequency of inundation of wetlands reduces the number of successful breeding and regeneration events in floodplain biota, causing a decline in the health and vigour of many floodplain plants and animals. The potential for rehabilitation of floodplain wetlands through increased flushing flows has been demonstrated. The ultimate aim is to enhance habitat value and achieve maximum biodiversity. Future opportunities are identified for hydrological manipulation within river operating strategies to extend beneficial management on a floodplain scale to improve wetland habitat. These can be combined with rehabilitation techniques such as specific water allocations, grazing controls and physical works on individual wetlands to ensure preservation and sustainable management of natural wetlands along the South Australian Murray Valley. Results so far indicate that successful restoration of the wetlands will take a number of flushing events and will be dependent primarily on the acceleration and protection of natural regeneration processes.