Biodegradation of phenol and monochlorophenols (CPs) by a yeast strain of Rhodotorula glutinis was examined. The strain completely degraded 5 mM of phenol and utilized phenol as a sole carbon source. The strain may degrade phenol by the “ortho” type of ring fission because muconolactone was observed in the cultured broth. 3-Chlorophenol (3-CP) and 4-chlorophenol (4-CP) were well degraded and stoichiometric release of chloride ion was observed, but degradation of 2-chlorophenol was low with a small quantity of chloride ions. Biodegradability of 3-CP and 4-CP was increased by the addition of phenol or CPs to the medium at the cell cultivation. Since 4-chlorocatechol and maleylacetic acid were determined as metabolites of 3-CP and 4-CP by GC/MS analysis, dechlorination may take place between the formation of 4-chlorocatechol and maleylacetic acid.
Biodegradation of phenol and monochlorophenols by yeast Rhodotorula glutinis
Keiko Katayama-Hirayama, Shusaku Tobita, Kimiaki Hirayama; Biodegradation of phenol and monochlorophenols by yeast Rhodotorula glutinis. Water Sci Technol 1 November 1994; 30 (9): 59–66. doi: https://doi.org/10.2166/wst.1994.0444
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