Reoviruses are known to be present in water of different origin. The family of the Reoviridae includes viruses which are infecting not only humans but also animals and even plants. A differentiation between the reovirus types becomes possible by comparison of the electrophoresis pattern of their segmented dsRNA genome. The following presents the profiles of virus isolates from different environmental water samples which have been concentrated and their nucleic acids purified and analysed by SDS-PAGE. Reoviruses were frequently found in different environmental water samples but also in drinking water samples. The most frequently isolated reovirus was the serotype 1 followed by the serotype 3. The serotype 2 was not found in the analysed samples.