This is a report of a field study based on data from an activated sludge process in a wastewater treatment plant in Chester, Pennsylvania, USA. The objective was to develop an accurate method for estimation of the average suspended solids concentration (SSB) of the layer of sludge in the settling tanks (the “sludge blanket”). Plant operators estimated SSB by averaging the mixed liquor suspended solids (Sm) and the return sludge suspended solids (Su) concentrations. Measurement of SSB showed that averaging Sm and Su frequently overestimated SSB by a large amount. A different relationship between SSB and parameters which are normally measured for operational purposes was developed. The parameters are Su, the overflow rates and data from the sludge volume index (SVI) measurement. It was found that an increasing overflow rate will result in an exponential decrease in the ratio of SSB to Su. Also, the SVI has a marked effect on the ratio of SSB to Su and thus on the amount of suspended solids which can be stored in the settling tanks. The proposed estimation equation was found to be statistically superior to estimation by averaging the Sm and Su.