Mechanisms of the anaerobic uptake of glutamate and aspartate by enhanced biological phosphorus removal activated sludge were investigated. Sludge hydrolysate with hydrochloric acid was analyzed by reverse phase HPLC after pre column derivatization with dabsyl-chloride. The experimental results indicated that glutamate is accumulated as a polymer consisting of γ-aminobutyric acid and an unknown amino acid. On the other hand, aspartate was found to be deaminated and then accumulated as PHA.

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