Capacitor oil samples (PCBs > 90%wt) were treated in a bench scale experiment to investigate the destruction of PCBs during chemical destruction processes (a catalytic hydrodechlorination treatment with palladium carbon and additional treatment with potassium tert-butyloxide). Using those results, this study confirmed the decrease of PCBs and other undesirable dioxin-like compounds such as PCDD/Fs in treated samples during the treatment. Dioxin-responsive chemical-activated luciferase expression (DR CALUX®) AhR reporter gene bioassay was used to evaluate dioxin-like activity in the samples. During the treatment, the efficiency for PCB capacitor oil was around 99.99% or more in WHO-TEQ and CALUX-TEQ, whereas the sum of PCBs was reduced at a resulting efficiency of >99.9999%. In this study, a new cleanup procedure for separating PCBs from the mineral oil matrix was also developed for DR CALUX. The procedure consists of dimethylsulphoxide partitioning followed by silica gel-44% sulphuric acid reflux treatment and activated carbon chromatography. With the cleanup, CALUX-TEQ values were in good agreement with WHO-TEQ values and were as much as 3.3 times higher than WHO-TEQs for untreated/treated PCB-containing insulating oil samples. The DR CALUX results of mineral oil samples containing various PCB concentrations of 0.5–50 mg/kg (corresponding WHO-TEQs: 0.012–1.2 μg-TEQ/g) also correlated well with WHO-TEQs (CALUX-TEQ/WHO-TEQ ratio =1.0–3.0), which was consistent with the theoretical quantification limit of the CALUX. These results supported the validity of the proposed clean-up method.
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Research Article|
May 01 2006
Bioassay monitoring of waste PCB samples during chemical destruction treatments
H. Takigami;
*Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan (E-mail: takigami@nies.go.jp)
E-mail: takigami@nies.go.jp
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P.A. Behnisch;
P.A. Behnisch
**Eurofins GfA, Otto-Hahn-Straße 22, D-48161, Münster-Roxel, Germany (E-mail: pbehnisch@gfa-ms.de)
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K. Shiozaki;
K. Shiozaki
***Kaneka Techno Research Co. Ltd., 1-8 Miyamae-machi, Takasago-cho, Takasago, Hyogo, 676-8688, Japan (E-mail: ken_shiozaki@kn.kaneka.co.jp)
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M. Ohno;
M. Ohno
****Kanden Engineering Co. Ltd., 3-1-176, Fukuzaki, Minato-ku, Osaka, 552-0013, Japan (E-mail: m-oono@kanden-eng.co.jp)
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S.-I. Sakai
S.-I. Sakai
*****Environment Preservation Center, Kyoto University, Yoshida Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan (E-mail: sakai@eprc.kyoto-u.ac.jp)
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Water Sci Technol (2006) 53 (11): 43–50.
Citation
H. Takigami, P.A. Behnisch, K. Shiozaki, M. Ohno, S.-I. Sakai; Bioassay monitoring of waste PCB samples during chemical destruction treatments. Water Sci Technol 1 May 2006; 53 (11): 43–50. doi: https://doi.org/10.2166/wst.2006.336
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H. Takigami, P.A. Behnisch, K. Shiozaki, M. Ohno, S.-I. Sakai; Bioassay monitoring of waste PCB samples during chemical destruction treatments. Water Sci Technol 1 May 2006; 53 (11): 43–50. doi: https://doi.org/10.2166/wst.2006.336
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