The simultaneous removal of H2S and NH3 was investigated using two biotrickling filters packed with polyurethane foam cubes. One biotrickling filter was inoculated with Thiobacillus thioparus ATCC 23645 for the removal of H2S (BTT) and the other filter with Nitrosomonas europaea ATCC 19718 for the removal of NH3 (BNE). Three different configurations were studied by modification of the gas line and recirculation medium line. The best results were obtained with the BNE biotrickling filter after the co-immobilization of the two bacteria. A removal efficiency of 100% for 230 ppmv of NH3 and 129 ppmv of H2S was reached at an EBRT of 60 seconds. The results obtained show that it is possible to co-immobilize both microorganisms using the same recirculation medium and remove successfully H2S and NH3 from a gas mixture.