Combined treatments were designed based on iron-carbon micro-electrolysis treatment (ICME), physical adsorption (PA) with zeolite (Z) or vermiculite (V) and microalgae cultivation (MC, Chlorella vulgaris) for removing pollutants from swine wastewater (SW): ICME + MC (IM), ICME + Z + MC (IZM) and ICME + V + MC (IVM). Results showed that the minimum total nitrogen (TN) of 43.66 mg L−1, NH4+-N of 1.33 mg−1 and total phosphorus (TP) of 0.14 mg−1 were obtained by IVM, while the minimum chemical oxygen demand (COD) was 105 mg−1 via IM. During the process of combined treatments, ICME contributed most to the removal of TN (84.52% by IZM), TP (97.78% by IVM and IZM) and COD (62.44% by IVM), and maximum NH4+-N removal (55.64%) was obtained by MC procedure in IM process. Vermiculite performed better than zeolite during all the combined treatments. Besides, the maximum cell dry weight (CDW, 0.74 g−1) of C. vulgaris was obtained by IM on day 13. The results provide an efficient integrated method for swine wastewater treatment.

  • Process combining iron-carbon microelectrolysis, vermiculite adsorption and microalgae cultivation was the most efficient for treatment on TN, NH4+-N, TP removal.

  • Iron carbon micro-electrolysis combined with microalgae cultivation was the most efficient treatment for COD removal.

  • The maximum biomass harvest was observed on day 13 via combined iron-carbon microelectrolysis and microalgae cultivation.

Large amount of swine wastewater (SW) is generated from concentrated animal feeding operations (CAFOs), leading to increasing concern for environmental safety. It was estimated that approximately 0.16 billion tons of SW is produced per year in China (Yu et al. 2020), which contains high concentrations of nutrients (nitrogen and phosphorus) and organic matter. If discharged untreated, SW would lead to environmental pollution such as contamination of soil, surface water, and ground water and the fresh water algal blooms. Furthermore, it increases the risks of human exposure to harmful pathogens and fish kill incidents (Cole et al. 2000; Smith & Schindler 2009; Damodara Kannan & Parameswaran 2021). Therefore, feasible and effective treatments are necessary before its discharge into the environment. Technologies such as biotreatment (anaerobic and aerobic), natural process (soil, lagoon, and wetland), physical treatment (adsorption and filtration) and chemical precipitation have been extensively applied worldwide for SW treatment (Szögi & Dept. Of Agriculture 2000; Chung et al. 2004; Huang et al. 2015). Different methods of treating SW and their removal efficiency are shown in Table 1. However, kinds of disadvantages such as high operational costs or occupation of too much land sources have been found associated with each single process during practical utilization. Thus, it was speculated that a combined process, capable of adopting the advantages of individual methods that could supplement each other, may be a more effective way to treat the aquaculture wastewater.

Table 1

Different methods of treating swine wastewater and their removal efficiency

MethodsTP removalCOD removalTN removalNH4+-N RemovalRefs.
Electrocoagulation 93% Mores et al. (2016)  
Microalgae cultivation of Tribonema sp. And Synechocystis sp. 71.4–72.7% 55.60% 75.8–89.9% Cheng et al. (2020b)  
Microalgae cultivation of Coelastrella sp. 74–78% 90–100% Luo et al. (2016)  
Chlorella vulgaris 60–70%% 40–90% Wang et al. (2015)  
Biofiltration 59% 90% 89% Kim et al. (2016)  
Aerobic 34.20% Inaba et al. (2018)  
Photosynthesis 83% Wen et al. (2016)  
Anoxic/aerobic process 75–86% 89–97% 80–94% Yang et al. (2016)  
Anaerobic digestion 65.8% Lourinho et al. (2020)  
MethodsTP removalCOD removalTN removalNH4+-N RemovalRefs.
Electrocoagulation 93% Mores et al. (2016)  
Microalgae cultivation of Tribonema sp. And Synechocystis sp. 71.4–72.7% 55.60% 75.8–89.9% Cheng et al. (2020b)  
Microalgae cultivation of Coelastrella sp. 74–78% 90–100% Luo et al. (2016)  
Chlorella vulgaris 60–70%% 40–90% Wang et al. (2015)  
Biofiltration 59% 90% 89% Kim et al. (2016)  
Aerobic 34.20% Inaba et al. (2018)  
Photosynthesis 83% Wen et al. (2016)  
Anoxic/aerobic process 75–86% 89–97% 80–94% Yang et al. (2016)  
Anaerobic digestion 65.8% Lourinho et al. (2020)  

COD, chemical oxygen demand; TN, total nitrogen; TP, total phosphorus.

Iron–carbon microelectrolysis (ICME) is a useful method for removal of wastewater pollutants. When a mixture of iron scraps and granular activated carbon (GAC) is in contact with SW (electrolyte solution), numerous microscopic galvanic cells are formed between the particles of iron (anode) and carbon (cathode) (Ying et al. 2012). The electrode reactions can be represented in terms of Equations (1) and (2) (Ju & Hu 2011) as follows:
(1)
(2)
Under aerating conditions in the ICME reactor, the oxygen competes as the electron acceptor and H2O2 is generated in situ via the reaction below (3):
(3)

The as-generated H2O2 subsequently combines with ferrous ions, released by dissolution of iron scraps, to form Fenton's reagent. It is a strong oxidizing agent that generates hydroxyl radical·OH through the well-known Fenton (Yao et al. 2020). Therefore, it is also referred to as the IME-Fenton reaction (Ying et al. 2012). The organic pollutants can be oxidized by radicals and can also be removed through adsorption, coprecipitation and enmeshment in the ferrous and ferric hydroxide floc (Cheng et al. 2007). The ICME method features low cost, simple operation, high efficiency, and low consumption, since it can remove refractory pollutants and improve the biodegradability of wastewater by changing the structure of some organic matters (Wang et al. 2016). It was reported that the ICME offers advantages in purifying wastewater containing high-concentration organic matter, and a chemical oxygen demand (COD) removal of 55% was achieved from an acrylonitrile–butadiene–styrene resin manufacturing process (Lai et al. 2012). Although there are many advantages of ICME for wastewater treatment, a single treatment procedure of ICME cannot meet the relevant need of nutrient removal. For example, the removal of ammonia nitrogen by ICME was reported as 15.5% (Liu et al. 2012). Therefore, further processing is undeniably required.

Moreover, it was found that ICME also exhibited poor performance toward total nitrogen (TN) removal (Zhang et al. 2014), which may can be expected to be covered by physical adsorption (PA). Zeolite (Z) has been widely used to remove ammonia and organic substances from wastewater owing to its low cost, abundance, simple operation, recyclability, and high adsorption capacity through ion exchange (Wang et al. 2011). Vermiculite (V) is well known for adsorption of ammonium and other impurities (Huo et al. 2012). A previous study revealed that zeolite, pumice, and sand can remove 13.3–75.2, 12.3–73.9, and 79–99% of total phosphorus (TP), respectively (Uzun et al. 2021). Huang et al. (2010) reported that Chinese zeolite can remove 92.6% of chemical oxygen demand (COD) and 80% of NH4+-N. Stefanakis & Tsihrintzis (2012) conducted an experiment and point out that bauxite can remove 54% of TP, 65% of COD, 35% of TN, and 38% of NH4+-N.

The utilization of wastewater as a nutrient source for microalgae cultivation (MC) is one of the best biological treatments, considering the significant reduction of nutrients (Luo et al. 2016) and valuable by-products of MC system (Cheng et al. 2020a). SW is a great nutrient source for MC because of its nutrient composition, comprising both the major nutrients and micronutrients (Zhang et al. 2016). However, the wastewater requires pretreatment before microalgae inoculation to reach the tolerance concentration of nutrients of microalgae (Cheng et al. 2020a). Employing the ICME or ICME + PA as pretreatment, a combined process of ICME + PA + MC could be a promising method for SW treatment.

In this study, a novel integrated treatment including ICME combined with PA and MC was proposed to investigate the efficiency of pollutants removal and biomass synthesis. The objective of this study was to figure out: (i) the efficiency of combined treatments on pollutants removal under the best optimized operating conditions; (ii) growth and biomass harvest of microalgae in SW.

Swine wastewater and preliminary treatment

The SW was collected from the experimental farm of Sichuan Agricultural University (SAU, Ya′an, Sichuan, China). Large non-soluble particulates in the SW were removed by sedimentation and filtration with gauze. Iron scraps were obtained from an agricultural machinery plant of Ya′an in China. Activated carbon, natural zeolite and vermiculite were purchased from the Qingyuan activated carbon factory of He′nan in China. The microalgae strain Chlorella vulgaris (C. vulgaris, FACHB-24) was purchased from Freshwater Algae Culture Collection (FACC) at the Institute of Hydrobiology (IH) of Wuhan, in China.

Before the experiment, the iron scraps were soaked in 10% sodium hydroxide solution for 2 h to remove oil from the surface. Activated carbon particles were soaked in SW for 48 hours and dried at 105 °C to eliminate the adsorbent effect. The zeolite (Z) and vermiculite (V) were washed three times with distilled water and then dried at 105 °C. The C. vulgaris strain was pre-cultivated in Blue-Green (BG11) medium until reaching the exponential growth phase for inoculation. Before C. vulgaris cultivation, the pH value of SW was adjusted to around 7.1.

Determination of optimized operating conditions

For ICME procedure, the iron scraps and activated carbon were mixed in the mass ratio of 1:1 and experiments were conducted at different dosages (50, 100, 150, 200, 250, 300, 400, and 450 g L−1), at different pH values (2.0, 3.0, 4.0, 5.0, 6.0, 7.0) for different times (0, 30, 60, 90, 120, 150, 180, 210, and 240 min) to optimize the operating conditions, and air was allowed to flow through the bottom of reaction devices (3 L min−1). Taking samples for water quality measurements, the best conditions for ICME treatment were determined as follows: mass of 200 g L−1, pH of 3, and 150 min in glass beakers with 200 mL SW.

For PA procedure, zeolite or vermiculite was added to wastewater (100 mL), which was then vibrated on a thermostat shaker at 150 rpm. The optimized operating conditions including mass of 350 g L−1 and 5 h were confirmed by pretreating at different dosages of zeolite or vermiculite (0, 50, 100, 150, 200, 250, 300, 350, and 400 g L−1) and adsorption times (0, 1, 2, 3, 4, 5, 6, 7, and 8 h).

Combined experiments

Three combined treatments were conducted to purify SW as shown in Figure 1. IM indicates that SW was treated by ICME and C. vulgaris cultivation, successively. IZM indicates that SW was treated by ICME, physical adsorption using zeolite and MC, successively. IVM indicates that SW was treated by ICME, PA using vermiculite, and MC, successively. For MC, C. vulgaris with a proportion of 20% (v/v) was inoculated in a 5 L flask. The cultivation condition was conducted at 25 ± 1 °C and under a continuous cool-white fluorescent light with an illumination of 3000–4000 Lx (12 L:12D). The air was allowed to flow through the bottom of the culture devices to agitate the algal broth as well as to supply carbon dioxide (1.5 L min−1 for first 6 days and 3 L min−1 for the remaining days for each flask). BG11 medium was used as control for measurement of microalgae growth. All experiments were performed in triplicate.

Figure 1

Design and schematic of experiment. (1) Swine wastewater; (2) iron particles and activated carbon particles; (3) natural zeolite; (4) vermiculite.

Figure 1

Design and schematic of experiment. (1) Swine wastewater; (2) iron particles and activated carbon particles; (3) natural zeolite; (4) vermiculite.

Close modal

Measurement of water quality parameters

The water samples were collected at the completion of ICME and PA procedures. The samples subjected to MC procedure were collected daily from each flask, then centrifuged at 8,000 rpm for 15 min, and the supernatant was taken for water quality measurement. The supernatant of each procedure was appropriately diluted for analyses of ammonium (NH4+-N), TN, and TP by ultraviolet spectrophotometry (Mapada UV-1200, Shanghai). The COD was measured using a Lovibond ET99722 multi-parameter water quality analyzer Manual (Lovibond, Germany). Nutrient removal rate (%) was calculated according to the method proposed by of Folino et al. (2020) by using the following equation:
(4)
where is the removal efficiency () of substrate i (NH4+-N, TN, TP or COD); and and are the initial and final concentrations of during the treatment, respectively.

Microalgae growth measurement

For microalgae growth analysis, samples (10 mL) were collected daily from each flask and dried in an oven at 105 °C (until constant weight) to evaluate microalgae cell dry weight (CDW) using a balance.

Data analysis

The experimental results were analyzed by using EXCEL (Microsoft Office Enterprise, 2010) and SPSS software (SPSS, v11.5). Statistically significant differences among means were determined by one-way analysis of variance (ANOVA) followed by Dunnett's test with significance at p < 0.05 for all tests.

Removal of total nitrogen from swine wastewater

Figure 2(a) exhibits that the TN decreases from 104 to 43.66–50.01 mg L−1 after combined treatments, and the maximum reduction was observed via IVM. Specifically, the ICME removed TN from 104 mg L−1 to 58.47 mg L−1, with the reduction rate of 43.67% and contribution rate of 75.37–84.52% by the three combined treatments. Lower TN removal rate (about 35%) of ICME was reported in a literature study (Lv et al. 2011) when Fe/C was utilized in the ratio of 1:1. This could be explained by the difference of the carbon particle size and initial pH between the two studies. The ICME procedure contributed most to the TN removal during combined treatments, because the iron ion, active hydrogen, and OH radicals generated in SW destabilized the colloidal pollutants, led to the occurrence of redox reaction, induced floc formation and dissolved compounds adsorption, and finally the pollutants were removed by flocculation and precipitation (Han et al. 2020).

Figure 2

(a) The TN of IM, IZM, and IVM. (b) Contribution rate of TN removal in IM, IZM, and IVM. (c) Removal rate of TN in IM, IZM and IVM during MC procedure.

Figure 2

(a) The TN of IM, IZM, and IVM. (b) Contribution rate of TN removal in IM, IZM, and IVM. (c) Removal rate of TN in IM, IZM and IVM during MC procedure.

Close modal

Besides, the PA removed TN from 58.47 to 45.37 mg L−1 (IZM) and 30.52 mg L−1 (IVM), with the reduction rate of 12.62% and 26.92%, respectively. A higher contribution rate of PA was observed in IVM (46.48%) than in IZM (24.43%) (Figure 2(b)). This result was attributed to stronger adsorption ability of vermiculite than zeolite toward TN removal. The effluent of TN includes total inorganic nitrogen (NH4+-N + NO3-N + NO2-N) and total organic nitrogen (TON) (Czerwionka et al. 2012). The zeolites have no affinity for anions because of the presence of permanent negative charge on their surface (Haron et al. 2008), which leads to a lower removal of TN for zeolite.

The MC procedure contributed 16.6% (IM), –8.95% (IZM), and –21.85% (IVM) toward TN removal during combined treatments (Figure 2(b)). Negative contribution was obtained due to the microalgae inoculation. The TN was increased by C. vulgaris solution from 58.47 to 80.44 mg L−1 via IM, from 45.37 to 73.14 mg L−1 via IZM, and from 30.52 to 56.77 mg L−1 via IM, as shown in Figure 2(a). The microalgae were inoculated into SW along with the medium solution (BG11), which contained organic nitrogen, leading to the increase of TN. At the completion of MC process, the removal rates of TN in IM, IZM, and IVM were 38.53, 31.39, and 22.85%, respectively (Figure 2(c)). The nitrogenous compounds in SW supplied nutrients to microalgae for their growth and proliferation (Stawiński et al. 2018), leading to the decrease of TN. TN of IM, IZM and IVM was 49.45, 50.17 and 43.66 mg L−1, respectively. IVM was significantly higher than IM and IZM (P<0.05) and there was no significant difference between IM and IZM (P>0.05).

Removal of NH4+-N in swine wastewater

Figure 3(a) demonstrates that concentration of NH4+-N decreased from 56.73 to 1.33–3.42 mg L−1 after combined treatments, and the maximum reduction was observed by IVM. Specifically, the ICME procedure reduced NH4+-N from 56.73 to 33.08 mg L−1, with the reduction rate of 41.69% and contribution rate of 42.69–44.36% in three combined treatments (Figure 3(a) and 3(b)). It was reported that the removal of ammonia nitrogen by ICME is a comprehensive process in which both physical and chemical adsorption are involved (Stawiński et al. 2018). Besides, the pH values of SW increased from 7.46 to 8.89 after ICME treatment (Table 2). This can be explained by the electrode reactions of ICME, where the hydrogen ions in cathode (carbon) were reduced and converted to hydrogen gas, causing the pH of the solution to increase (Yang et al. 2009) and thus indicating the increase in the amount of hydroxyl groups during ICME.

Table 2

pH values of swine wastewater

ProcedurepH
Raw 7.46 ± 0.05 
After ICME 8.89 ± 0.12 
After adsorption with zeolite 7.70 ± 0.47 
After adsorption with vermiculite 7.89 ± 0.58 
ProcedurepH
Raw 7.46 ± 0.05 
After ICME 8.89 ± 0.12 
After adsorption with zeolite 7.70 ± 0.47 
After adsorption with vermiculite 7.89 ± 0.58 
Figure 3

(a) The NH4+-N of IM, IZM, and IVM. (b) Contribution rate of NH4+-N removal in IM, IZM, and IVM.

Figure 3

(a) The NH4+-N of IM, IZM, and IVM. (b) Contribution rate of NH4+-N removal in IM, IZM, and IVM.

Close modal

The PA led to the decrease in the amount of NH4+-N from 33.08 to 21.58 mg L−1 and 5.6 mg L−1 via IZM and IVM, with the reduction rate of 20.27 and 48.44%, respectively (Figure 3(a)). A higher contribution rate of PA was observed by IVM (49.6%) than that by IZM (22.09%) (Figure 3(b)). According to literature (Fan et al. 2021), the removal of ammonia nitrogen by zeolite reached up to 66.97%, and that was much higher than the removal rate of 20.27% obtained in this study. It was mainly due to the alkaline initial pH value of PA procedure (8.89, Table 2), and the alkalinity of solution was adverse for ammonia removal. The previous study revealed that weak acidic condition was favorable for ammonia nitrogen removal and the ammonia nitrogen reduction by zeolite began to decline when pH of wastewater was above 6.18 (Fan et al. 2021), and it decreased sharply when pH of solution was above 8 (Huang et al. 2010). Thus, according to the previous studies, the NH4+-N removal rate of vermiculite increased slightly when pH was in the range of 6–10 and could reach up to 73% when pH was 9 (Huang et al. 2010). Therefore, NH4+-N adsorption ability of vermiculite was stronger than that of zeolite under such an alkaline condition of this experiment.

The MC procedure contributed 55.64%, 33.63% and 7.71% in IM, IZM and IVM, respectively (Figure 3(b)) to NH4+-N removal during combined treatments. According to previous study, ammonia nitrogen could be assimilated into algal cells in the liquid as well as a portion of ammonia being trapped in the liquid due to its solubility (Kang & Wen 2015). As a result, the concentration of NH4+-N in three groups declined and the difference of removal rate among the three treatments was due to the different initial NH4+-N content during MC. The concentration of effluent NH4+-N of IM, IZM and IVM was 3.42, 3.32 and 1.33 mg L−1, respectively.

Removal of total phosphorus in swine wastewater

The TP decreased from 48.29 to 0.14–0.15 mg L−1 after combined treatments, and the maximum reduction was obtained via IVM (Figure 4(a)). Specifically, the ICME reduced TP from 48.29 to 1.22 mg L−1, with the reduction rate of 97.47% and contribution rate of 97.76–97.78% in three combined treatments (Figure 4(b)). A similar study was also reported by Shen et al. (2019), who found that ICME removed 93.63% of TP in a constructed wetland system. It was revealed that ICME involved the generation of hydrogen, ferrous ions, ferric ions and hydroxide (Yang et al. 2009; Zhang et al. 2018). The hydrogen gas contributed to flotation of the flocculated particles out of wastewater (Braun et al. 2019). The iron scraps of ICME were the main reason for TP removal, because phosphorus ions have strong affinity for Fe3+. Oxides of Fe in aqueous medium consist of surface OH groups, which can cause surface adsorption of P by complexation (Stawiński et al. 2018).

Figure 4

(a) The TP of IM, IZM, and IVM. (b) Contribution rate of TP removal in IM, IZM, and IVM.

Figure 4

(a) The TP of IM, IZM, and IVM. (b) Contribution rate of TP removal in IM, IZM, and IVM.

Close modal

The PA removed TP from 1.22 to 0.54 mg L−1 and 0.59 mg L−1 with the reduction of rate of 1.41% and 1.3% by IZM and IVM, respectively (Figure 4(a)). The TP decreased in IVM owing to the adsorption by vermiculite (Zhao et al. 2016), and in IZM owing to both adsorption and precipitation of phosphate by zeolite (Karapınar 2009). Therefore, the reduction of TP by zeolite was slightly higher than that by vermiculite.

The MC procedure contributed to TP removal of 2.22% (IM), 0.81% (IZM) and 0.93% (IVM) during combined treatments (Figure 4(b)). Phosphorus is an essential element for the microalgae growth and proliferation, and it can be assimilated and accumulated into the microalgae biomass (Karpagam et al. 2015). The effluent TP contents in IM, IZM, and IVM were 0.15, 0.15, and 0.14 mg L−1.

Removal of chemical oxygen demand in swine wastewater

The COD decreased from 1,880 mg/L to 105–116.67 mg L−1 after combined treatments, and no significant difference (P>0.05) was observed among IM, IZM, and IVM (Figure 5(a)). Specifically, the ICME procedure reduced COD from 1,880 to 779 mg L−1, with the reduction rate of 58.56% and contribution rate of 62.03–62.44% (Figure 5(b)) in three combined treatments. Higher COD reduction by ICME was found in this study compared with the literature report (Ma et al. 2019) (removal rate of 27.61%). This result may be attributed to higher dosage of iron–carbon system utilized in this experiment. The proportion of iron and carbon utilized in this experiment was 0.2, while the counterpart was 0.06 in the literature study (Ma et al. 2019).

Figure 5

(a) The COD of IM, IZM, and IVM. (b) Contribution rate of COD removal in IM, IZM, and IVM.

Figure 5

(a) The COD of IM, IZM, and IVM. (b) Contribution rate of COD removal in IM, IZM, and IVM.

Close modal

The COD removal rate by PA procedure was inefficient. It contributed –0.04% and –1.27% via IZM and IVM, respectively (Figure 5(b)). According to literature, a substance with hydrophobic surfaces is more suitable for adsorption of organic substances (Yang et al. 2020). The surface of zeolite is hydrophilic (Halim et al. 2010), and the hydrophilic nature of vermiculite also hindered its adsorption capacity toward the removal of organic contaminants (Mujtaba et al. 2018). This can explain the poor adsorption characterization of zeolite and vermiculite to organic material.

The effluent COD after MC procedure was 105, 110.33, and 116.67 mg L−1 in IM, IZM, and IVM, respectively (Figure 5(a)). IVM was significantly higher than IM (P<0.05) and there was no significant difference between IVM and IZM (P>0.05) and no significant between IZM and IM(P>0.05). No significant difference (P>0.05) on removal rate of COD was found among IM (35.85%), IZM (35.60%), and IVM (36.41%), and contribution rates of IM (37.97%), IZM (37.79%), and IVM (37.56%) (Figure 5(b)). The higher COD removal rate (72.6%) was found by Wang et al. (2015)'s research, where the initial ammonia nitrogen was 80 mg L−1. However, initial ammonia nitrogen of MC was 33.08 mg L−1 (IM), 21.58 mg L−1 (IZM), and 5.6 mg L−1 (IVM) in this study. Lack of nitrogen sources can trigger the secretion of extracellular organic matter (EOM), leading to the increase of COD (Wang et al. 2015). Moreover, the higher contents of initial nitrogen and phosphorus will promote the growth of microalgae, leading to a higher COD removal (Scarponi et al. 2021).

Growth of C. vulgaris

The biomass of C. vulgaris was analyzed in four groups (Figure 6). The reduced CDWs on day 2 were related to the flocculation and precipitation caused by suspended impurities along with cells in wastewater. The CDWs in IM showed an increasing trend, while those in IVM and IZM showed a decreasing trend. The result indicated there were not sufficient nutrients (TN, TP, and NH4+-N) in IVM and IZM to support growth of C. vulgaris, which led to the limited growth of microalgae (Vargas-Estrada et al. 2021). Minimal nutritional requirements can be estimated by the approximate molecular formula (CO0.48H1.83N0.11 P0.01) of the microalgal biomass (Chisti 2007). Although accounting for only 1%, phosphorus is often one of the most important growth-limiting factors (Ferreira et al. 2021). It can be adsorbed into cells by microalgae and then participate in the process of energy synthesis through a variety of phosphorylation processes such as substrate level phosphorylation, oxidative phosphorylation, and photosynthetic phosphorylation (Wang et al. 2021). In this study, 3 mg L−1 of phosphorus was required for microalgae (CDW of 0.3 g L−1) of IM on day 2. However, the content of TP was 0.54 and 0.59 mg L−1 in IZM and IVM, respectively, after the PA process, which led to the decline of CDWs of algae. After 13-day MC, the CDW of IM started to decline, and CDW of IM dropped to 0.42 g L−1 on day 16. The maximal CDW (0.74 g L−1) was observed in IM on day 13, and it was much higher than those in other treatments, including control. This may be attributed to the effective electrode transfer after ICME stimulated microbial growth and metabolic enzyme activity, and further promoted biodegradation ability of microorganisms (Zhang et al. 2012). Besides, on day 13, the TP, NH4+-N and COD in IM were 0.15, 6.7, and 116 mg L−1, and there was no significant difference (P>0.05) between day 13 and day 16. Therefore, for both purification of SW and harvest of biomass, ICME procedure integrated with MC for 13 days was the best option among three combined treatments.

Figure 6

CDW of C. vulgaris during the culture in swine wastewater.

Figure 6

CDW of C. vulgaris during the culture in swine wastewater.

Close modal

Reusability and cost of adsorbents

According to the previous studies (Halim et al. 2010), the reusability of regenerated zeolite was better than that of the fresh one toward ammoniacal nitrogen. Moreover, the ammoniacal nitrogen and COD removal by regenerated zeolite increased by 7.3% and decreased by 2.6%, respectively (Halim et al. 2010). Fan et al. (2021) reported the effect of the zeolites, which remained stable after reuse for three times. In general, 21 g of ammonium nitrogen can be adsorbed by 21 kg of vermiculite within 2 h; however, the removal efficiency of vermiculite dropped to 52 and 38% after 3 and 5 h, respectively (Rama et al. 2019). Zhang et al. (2021) conducted an experiment and pointed out that removal efficiency of biochar drops to 71.3% after three regeneration cycles. It can be concluded from above findings that the biochar, zeolite and vermiculite can be reused for three or two times at least for ammonia nitrogen removal.

The market unit price of natural zeolite, vermiculite and biochar was $50–300 (Szerement et al. 2021), $850 (Brião et al. 2021) and $2062–2512 (Campbell et al. 2018), respectively. The corresponding cost for removing 1,000 g ammonia nitrogen was investigated and the smallest cost was $13 for zeolite (Table 3).

Table 3

Costs of removal per 1,000 g of ammonia nitrogen

MaterialRegeneration timesChangeCapacity of adoption (fresh)Total ammonia adsorption by 2 or 3 cycles (g/kg)Requirements per 1,000 g NH4+-N(kg)Price (kg)Cost ($)
Zeolite Stable 4.46 mg/g 13.38 74.74 $0.175 13.08 
Vermiculite Drop to 52% after 3 h 1.75 mg/g 3.5 285.71 $0.85 242.86 
Biochar Drop to 71.3% 8.5 mg/g 21.84 45.79 $2.287 104.71 
MaterialRegeneration timesChangeCapacity of adoption (fresh)Total ammonia adsorption by 2 or 3 cycles (g/kg)Requirements per 1,000 g NH4+-N(kg)Price (kg)Cost ($)
Zeolite Stable 4.46 mg/g 13.38 74.74 $0.175 13.08 
Vermiculite Drop to 52% after 3 h 1.75 mg/g 3.5 285.71 $0.85 242.86 
Biochar Drop to 71.3% 8.5 mg/g 21.84 45.79 $2.287 104.71 

All the combined treatments IM, IZM, and IVM can remove pollutants in swine wastewater. The minimum effluent TN, NH4+-N, and TP was obtained via IVM, and the minimum effluent COD was observed in IM. During the combined treatments, ICME contributed the most to the TN (84.52% in IZM), TP (97.78% in IVM and IZM), and COD (62.44% in IVM), while MC contributed the most to the NH4+-N removal (55.64%). Vermiculite performed better than zeolite during all the combined treatments. Moreover, the maximum biomass harvest was obtained via IM. The results provide useful information for developing efficient and economical methods to remove pollutants from swine wastewater. Undeniably, a lot more systematic explorations are still demanded to investigate the removal of more pollutants such as heavy metals, antibiotic and hormones; this should be explored by combined treatment that will be pursued in the near future.

This research was financially supported by the National Natural Science Foundation of China (31702156), the Sichuan Swine Innovation Team Construction Project of National Modern Agricultural Industry Technology System of China (sccxtd-2021-08), and Chongqing & Rongchang Agriculture and Animal Husbandry High-tech Industry Research and Development Special Project (cstc2019ngzx0004).

All relevant data are included in the paper or its Supplementary Information.

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